diff --git a/jerry-core/ecma/builtin-objects/ecma-builtin-object-prototype.c b/jerry-core/ecma/builtin-objects/ecma-builtin-object-prototype.c
index dd0bdca45..9044e9b95 100644
--- a/jerry-core/ecma/builtin-objects/ecma-builtin-object-prototype.c
+++ b/jerry-core/ecma/builtin-objects/ecma-builtin-object-prototype.c
@@ -51,7 +51,11 @@ enum
   ECMA_OBJECT_PROTOTYPE_IS_PROTOTYPE_OF,
   ECMA_OBJECT_PROTOTYPE_HAS_OWN_PROPERTY,
   ECMA_OBJECT_PROTOTYPE_PROPERTY_IS_ENUMERABLE,
-  ECMA_OBJECT_PROTOTYPE_SET_PROTO
+  ECMA_OBJECT_PROTOTYPE_SET_PROTO,
+#if ENABLED (JERRY_ESNEXT) && ENABLED (JERRY_BUILTIN_ANNEXB)
+  ECMA_OBJECT_PROTOTYPE_DEFINE_GETTER,
+  ECMA_OBJECT_PROTOTYPE_DEFINE_SETTER,
+#endif /* ENABLED (JERRY_ESNEXT) && ENABLED (JERRY_BUILTIN_ANNEXB) */
 };
 
 #define BUILTIN_INC_HEADER_NAME "ecma-builtin-object-prototype.inc.h"
@@ -203,6 +207,63 @@ ecma_builtin_object_prototype_object_property_is_enumerable (ecma_object_t *obj_
   return ecma_make_boolean_value (is_enumerable);
 } /* ecma_builtin_object_prototype_object_property_is_enumerable */
 
+#if ENABLED (JERRY_ESNEXT) && ENABLED (JERRY_BUILTIN_ANNEXB)
+/**
+ * The Object.prototype object's '__defineGetter__' and '__defineSetter__' routine
+ *
+ * See also:
+ *          ECMA-262 v11, B.2.2.2
+ *          ECMA-262 v11, B.2.2.3
+ *
+ * @return ECMA_VALUE_ERROR - if the operation fails,
+ *         ECMA_VALUE_UNDEFINED - otherwise
+ */
+static ecma_value_t
+ecma_builtin_object_prototype_define_getter_setter (ecma_object_t *obj_p, /**< this argument */
+                                                    ecma_string_t *prop_name_p, /**< property name */
+                                                    ecma_value_t accessor, /**< getter/setter function */
+                                                    bool define_getter) /**< true - defineGetter method
+                                                                             false - defineSetter method */
+{
+  /* 2. */
+  if (!ecma_op_is_callable (accessor))
+  {
+    return ecma_raise_type_error (ECMA_ERR_MSG ("Getter is not callable."));
+  }
+
+  ecma_object_t *accessor_obj_p = ecma_get_object_from_value (accessor);
+
+  /* 3. */
+  ecma_property_descriptor_t desc = ecma_make_empty_property_descriptor ();
+  desc.flags |= (ECMA_PROP_IS_ENUMERABLE
+                 | ECMA_PROP_IS_CONFIGURABLE
+                 | ECMA_PROP_IS_ENUMERABLE_DEFINED
+                 | ECMA_PROP_IS_CONFIGURABLE_DEFINED);
+
+  if (define_getter)
+  {
+    desc.get_p = accessor_obj_p;
+    desc.flags |= ECMA_PROP_IS_GET_DEFINED;
+  }
+  else
+  {
+    desc.set_p = accessor_obj_p;
+    desc.flags |= ECMA_PROP_IS_SET_DEFINED;
+  }
+
+  /* 5. */
+  ecma_value_t define_prop = ecma_op_object_define_own_property (obj_p, prop_name_p, &desc);
+
+  if (ECMA_IS_VALUE_ERROR (define_prop))
+  {
+    return define_prop;
+  }
+
+  /* 6. */
+  return ECMA_VALUE_UNDEFINED;
+} /* ecma_builtin_object_prototype_define_getter_setter */
+#endif /* ENABLED (JERRY_ESNEXT) && ENABLED (JERRY_BUILTIN_ANNEXB) */
+
 /**
  * Dispatcher of the built-in's routines
  *
@@ -307,6 +368,16 @@ ecma_builtin_object_prototype_dispatch_routine (uint16_t builtin_routine_id, /**
   {
     ret_value = ecma_builtin_object_prototype_object_has_own_property (obj_p, prop_name_p);
   }
+#if ENABLED (JERRY_ESNEXT) && ENABLED (JERRY_BUILTIN_ANNEXB)
+  else if (builtin_routine_id == ECMA_OBJECT_PROTOTYPE_DEFINE_GETTER)
+  {
+    ret_value = ecma_builtin_object_prototype_define_getter_setter (obj_p, prop_name_p, arguments_list_p[1], true);
+  }
+  else if (builtin_routine_id == ECMA_OBJECT_PROTOTYPE_DEFINE_SETTER)
+  {
+    ret_value = ecma_builtin_object_prototype_define_getter_setter (obj_p, prop_name_p, arguments_list_p[1], false);
+  }
+#endif /* ENABLED (JERRY_ESNEXT) && ENABLED (JERRY_BUILTIN_ANNEXB) */
   else
   {
     ret_value = ecma_builtin_object_prototype_object_property_is_enumerable (obj_p, prop_name_p);
diff --git a/jerry-core/ecma/builtin-objects/ecma-builtin-object-prototype.inc.h b/jerry-core/ecma/builtin-objects/ecma-builtin-object-prototype.inc.h
index fccab0ff1..86e38c327 100644
--- a/jerry-core/ecma/builtin-objects/ecma-builtin-object-prototype.inc.h
+++ b/jerry-core/ecma/builtin-objects/ecma-builtin-object-prototype.inc.h
@@ -41,4 +41,9 @@ ROUTINE (LIT_MAGIC_STRING_HAS_OWN_PROPERTY_UL, ECMA_OBJECT_PROTOTYPE_HAS_OWN_PRO
 ROUTINE (LIT_MAGIC_STRING_IS_PROTOTYPE_OF_UL, ECMA_OBJECT_PROTOTYPE_IS_PROTOTYPE_OF, 1, 1)
 ROUTINE (LIT_MAGIC_STRING_PROPERTY_IS_ENUMERABLE_UL, ECMA_OBJECT_PROTOTYPE_PROPERTY_IS_ENUMERABLE, 1, 1)
 
+#if ENABLED (JERRY_ESNEXT) && ENABLED (JERRY_BUILTIN_ANNEXB)
+ROUTINE (LIT_MAGIC_STRING_DEFINE_GETTER, ECMA_OBJECT_PROTOTYPE_DEFINE_GETTER, 2, 2)
+ROUTINE (LIT_MAGIC_STRING_DEFINE_SETTER, ECMA_OBJECT_PROTOTYPE_DEFINE_SETTER, 2, 2)
+#endif /* ENABLED (JERRY_ESNEXT) && ENABLED (JERRY_BUILTIN_ANNEXB) */
+
 #include "ecma-builtin-helpers-macro-undefs.inc.h"
diff --git a/jerry-core/lit/lit-magic-strings.inc.h b/jerry-core/lit/lit-magic-strings.inc.h
index e9893ec8a..9d53e7c9c 100644
--- a/jerry-core/lit/lit-magic-strings.inc.h
+++ b/jerry-core/lit/lit-magic-strings.inc.h
@@ -879,6 +879,10 @@ LIT_MAGIC_STRING_DEF (LIT_MAGIC_STRING_SET_MILLISECONDS_UL, "setMilliseconds")
 LIT_MAGIC_STRING_DEF (LIT_MAGIC_STRING_MAX_SAFE_INTEGER_U, "MAX_SAFE_INTEGER")
 LIT_MAGIC_STRING_DEF (LIT_MAGIC_STRING_MIN_SAFE_INTEGER_U, "MIN_SAFE_INTEGER")
 #endif
+#if ENABLED (JERRY_ESNEXT) && ENABLED (JERRY_BUILTIN_ANNEXB)
+LIT_MAGIC_STRING_DEF (LIT_MAGIC_STRING_DEFINE_GETTER, "__defineGetter__")
+LIT_MAGIC_STRING_DEF (LIT_MAGIC_STRING_DEFINE_SETTER, "__defineSetter__")
+#endif
 LIT_MAGIC_STRING_DEF (LIT_MAGIC_STRING_DEFINE_PROPERTIES_UL, "defineProperties")
 #if ENABLED (JERRY_BUILTIN_TYPEDARRAY)
 LIT_MAGIC_STRING_DEF (LIT_MAGIC_STRING_BYTES_PER_ELEMENT_U, "BYTES_PER_ELEMENT")
@@ -1057,11 +1061,15 @@ LIT_MAGIC_STRING_FIRST_STRING_WITH_SIZE (15, LIT_MAGIC_STRING_STRING_ITERATOR_UL
 LIT_MAGIC_STRING_FIRST_STRING_WITH_SIZE (15, LIT_MAGIC_STRING_GET_MILLISECONDS_UL)
 #elif ENABLED (JERRY_BUILTIN_NUMBER) && ENABLED (JERRY_ESNEXT)
 LIT_MAGIC_STRING_FIRST_STRING_WITH_SIZE (15, LIT_MAGIC_STRING_MAX_SAFE_INTEGER_U)
+#elif ENABLED (JERRY_ESNEXT) && ENABLED (JERRY_BUILTIN_ANNEXB)
+LIT_MAGIC_STRING_FIRST_STRING_WITH_SIZE (15, LIT_MAGIC_STRING_DEFINE_GETTER)
 #else
 LIT_MAGIC_STRING_FIRST_STRING_WITH_SIZE (15, LIT_MAGIC_STRING_DEFINE_PROPERTIES_UL)
 #endif
 #if ENABLED (JERRY_BUILTIN_NUMBER) && ENABLED (JERRY_ESNEXT)
 LIT_MAGIC_STRING_FIRST_STRING_WITH_SIZE (16, LIT_MAGIC_STRING_MAX_SAFE_INTEGER_U)
+#elif ENABLED (JERRY_ESNEXT) && ENABLED (JERRY_BUILTIN_ANNEXB)
+LIT_MAGIC_STRING_FIRST_STRING_WITH_SIZE (16, LIT_MAGIC_STRING_DEFINE_GETTER)
 #else
 LIT_MAGIC_STRING_FIRST_STRING_WITH_SIZE (16, LIT_MAGIC_STRING_DEFINE_PROPERTIES_UL)
 #endif
diff --git a/jerry-core/lit/lit-magic-strings.ini b/jerry-core/lit/lit-magic-strings.ini
index 069648230..26e379372 100644
--- a/jerry-core/lit/lit-magic-strings.ini
+++ b/jerry-core/lit/lit-magic-strings.ini
@@ -353,6 +353,8 @@ LIT_MAGIC_STRING_SET_MILLISECONDS_UL = "setMilliseconds"
 LIT_MAGIC_STRING_DEFINE_PROPERTIES_UL = "defineProperties"
 LIT_MAGIC_STRING_MAX_SAFE_INTEGER_U = "MAX_SAFE_INTEGER"
 LIT_MAGIC_STRING_MIN_SAFE_INTEGER_U = "MIN_SAFE_INTEGER"
+LIT_MAGIC_STRING_DEFINE_GETTER = "__defineGetter__"
+LIT_MAGIC_STRING_DEFINE_SETTER = "__defineSetter__"
 LIT_MAGIC_STRING_BYTES_PER_ELEMENT_U = "BYTES_PER_ELEMENT"
 LIT_MAGIC_STRING_NEGATIVE_INFINITY_U = "NEGATIVE_INFINITY"
 LIT_MAGIC_STRING_POSITIVE_INFINITY_U = "POSITIVE_INFINITY"
diff --git a/tests/jerry/es.next/object-prototype-define-getter.js b/tests/jerry/es.next/object-prototype-define-getter.js
new file mode 100644
index 000000000..ae2de6d60
--- /dev/null
+++ b/tests/jerry/es.next/object-prototype-define-getter.js
@@ -0,0 +1,51 @@
+// Copyright JS Foundation and other contributors, http://js.foundation
+//
+// Licensed under the Apache License, Version 2.0 (the "License");
+// you may not use this file except in compliance with the License.
+// You may obtain a copy of the License at
+//
+//     http://www.apache.org/licenses/LICENSE-2.0
+//
+// Unless required by applicable law or agreed to in writing, software
+// distributed under the License is distributed on an "AS IS" BASIS
+// WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
+// See the License for the specific language governing permissions and
+// limitations under the License.
+
+var o = {};
+o.__defineGetter__('foo', function() { return 5; });
+assert(o.foo === 5);
+
+var obj = {};
+function bar() { return "bar"; }
+Object.prototype.__defineGetter__.call(obj, "foo", bar);
+var prop = Object.getOwnPropertyDescriptor(obj, "foo");
+assert(prop.get == bar);
+assert(!prop.writable);
+assert(prop.configurable);
+assert(prop.enumerable);
+
+var obj = {};
+var sym = Symbol();
+function bar() { return "bar"; }
+Object.prototype.__defineGetter__.call(obj, sym, bar);
+var prop = Object.getOwnPropertyDescriptor(obj, sym);
+assert(prop.get == bar);
+assert(!prop.writable);
+assert(prop.configurable);
+assert(prop.enumerable);;
+
+var key = '__accessors_test__';
+Object.prototype.__defineGetter__.call(1, key, function(){});
+
+try {
+  Object.prototype.__defineGetter__.call(null, key, function(){});
+  assert(false);
+} catch(e){
+  assert(e instanceof TypeError);
+}
+
+var def = [];
+var p = new Proxy({}, { defineProperty: function(o, v, desc) { def.push(v); Object.defineProperty(o, v, desc); return true; }});
+Object.prototype.__defineGetter__.call(p, "foo", Object);
+assert(def + '' === "foo");
diff --git a/tests/jerry/es.next/object-prototype-define-setter.js b/tests/jerry/es.next/object-prototype-define-setter.js
new file mode 100644
index 000000000..cea8d6251
--- /dev/null
+++ b/tests/jerry/es.next/object-prototype-define-setter.js
@@ -0,0 +1,53 @@
+// Copyright JS Foundation and other contributors, http://js.foundation
+//
+// Licensed under the Apache License, Version 2.0 (the "License");
+// you may not use this file except in compliance with the License.
+// You may obtain a copy of the License at
+//
+//     http://www.apache.org/licenses/LICENSE-2.0
+//
+// Unless required by applicable law or agreed to in writing, software
+// distributed under the License is distributed on an "AS IS" BASIS
+// WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
+// See the License for the specific language governing permissions and
+// limitations under the License.
+
+var o = {};
+o.__defineSetter__('foo', function(val) { this.bar = val; });
+o.foo = 5;
+assert(o.foo === undefined); // undefined
+assert(o.bar === 5); 
+
+var obj = {};
+function bar() {}
+Object.prototype.__defineSetter__.call(obj, "foo", bar);
+var prop = Object.getOwnPropertyDescriptor(obj, "foo");
+assert(prop.set === bar);
+assert(!prop.writable);
+assert(prop.configurable);
+assert(prop.enumerable);
+
+var obj = {};
+var sym = Symbol();
+function bar(baz) {}
+Object.prototype.__defineSetter__.call(obj, sym, bar);
+var prop = Object.getOwnPropertyDescriptor(obj, sym);
+assert(prop.set === bar);
+assert(!prop.writable);
+assert(prop.configurable);
+assert(prop.enumerable);
+
+var key = '__accessors_test__';
+Object.prototype.__defineSetter__.call(1, key, function(){});
+
+try {
+  Object.prototype.__defineSetter__.call(null, key, function(){});
+  assert(false);
+} catch (e) {
+  assert(e instanceof TypeError);
+}
+
+var def = [];
+var p = new Proxy({}, { defineProperty: function(o, v, desc) { def.push(v); Object.defineProperty(o, v, desc); return true; }});
+Object.prototype.__defineSetter__.call(p, "foo", Object);
+assert(def + '' === "foo");